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SAB4200274

Sigma-Aldrich

Anti-EIF2C2/Ago2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-Eukaryotic translation initiation factor 2C, 2, Anti-I2C2, Argonaute-2, Anti-PAZ Piwi domain protein, Anti-Slicer protein

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~97 kDa

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human EIF2C2/Ago2

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... EIF2C2(27161)
mouse ... Eif2c2(239528)
rat ... Eif2c2(59117)

Description générale

Anti-eukaryotic translation initiation factor 2C 2 (EIF2C2)/protein argonaute-2 (Ago3) is produced in rabbit using as immunogen a synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2 conjugated to KLH. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. Ago subfamily consists of hsAgo1-4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies.

Immunogène

synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

Ago2 has endonuclease activity, that cleaves the target RNA in RNAi. Ago2 is regulated at both the transcriptional and posttranslational levels in human breast cancer cell lines. It is implicated in micro-RNA activity and in the tumorigenic progression of breast cancer cell lines.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells
Adams BD, et al.
Endocrinology, 150(1), 14-23 (2008)
Argonaute proteins: mediators of RNA silencing
Peters L and Meister G
Molecular Cell, 26(5), 611-623 (2007)
Terence N Bukong et al.
PLoS pathogens, 10(10), e1004424-e1004424 (2014-10-03)
Antibodies targeting receptor-mediated entry of HCV into hepatocytes confer limited therapeutic benefits. Evidence suggests that exosomes can transfer genetic materials between cells; however, their role in HCV infection remains obscure. Here, we show that exosomes isolated from sera of chronic
The Argonaute protein family
Hock J and Gunter M
Genome Biology, 9(2), 210-210 (2008)

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