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S9571

Sigma-Aldrich

Anti-SUMO 2 antibody produced in rabbit

enhanced validation

~0.6 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-SMT3B, Anti-SMT3H2, Anti-Sentrin-2

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About This Item

Numéro MDL:
MFCD03455941
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~15 kDa

Espèces réactives

human

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Concentration

~0.6 mg/mL

Technique(s)

indirect immunofluorescence: 10-20 μg/mL using HeLa cells
western blot: 0.5-1 μg/mL using nuclear extract of HeLa cells or cell extract of HEK293-T cells transfected with human SUMO-2

Numéro d'accès UniProt

P61956

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SUMO2(6613)
mouse ... Sumo2(170930)

Description générale

Small ubiquitin-related modifier (SUMO-2) gene is mapped to human chromosome 17q25.1. Sumo-2 is related to SUMO-3 with a 86% sequence identity. It comprises a sumoylation motif at the residue lysine 11.

Spécificité

Anti-SUMO-2 recognizes human SUMO-2.

Immunogène

synthetic peptide corresponding to amino acids 80-93 located at the C-terminal of human SUMO-2, conjugated to KLH. This sequence is identical in many species including rat, mouse, pig, and bovine SUMO-2 and is identical in human SUMO-3.

Application

Anti-SUMO-2 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

Small ubiquitin-related modifier (SUMO-2) is essential for the cellular defense in hyperthermic cytotoxicity. Along with SUMO-3, SUMO2 is essential for cell survival post-heat shock. SUMO-2 has the ability to exist as self-modified polymers. SUMO-2 and SUMO-3 negatively regulate interferon induction.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 1% bovien serum albumin and 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

John T Crowl et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(26), 6798-6803 (2018-06-13)
Detection of nucleic acids by innate immune sensors triggers the production of type I interferons (IFNs). While IFNs are essential for host defense against viral infection, dysregulated production of IFNs underlies numerous autoinflammatory diseases. We have found that the loss
Hong-Lin Su et al.
Gene, 296(1-2), 65-73 (2002-10-18)
The SUMO (small ubiquitin-like modifier) protein and ubiquitin have similar 3-D structure. Sumolyzation and ubiquitination exhibit similar biological processes for post-translational modification. However, unlike ubiquitination, which targets proteins for degradation, sumolyzation participates in a number of cellular processes such as
Rajarshi Chakrabarti et al.
Biochemical and biophysical research communications, 439(2), 209-214 (2013-09-03)
Phosphatidylinositol 4 phosphate 5 kinase 1α (PIP5K) is mainly localized in the cytosol and plasma membrane. Studies have also indicated its prominent association with nuclear speckles. The exact nature of this nuclear pool of PIP5K is not clear. Using biochemical
Filip Golebiowski et al.
Science signaling, 2(72), ra24-ra24 (2009-05-28)
Covalent conjugation of the small ubiquitin-like modifier (SUMO) proteins to target proteins regulates many important eukaryotic cellular mechanisms. Although the molecular consequences of the conjugation of SUMO proteins are relatively well understood, little is known about the cellular signals that
A B Steffensen et al.
Acta physiologica (Oxford, England), 222(3) (2017-09-10)
The voltage-gated potassium channel KV 11.1 is the molecular basis for the IKr current, which plays an important role in cardiac physiology. Its malfunction is associated with both inherited and acquired cardiac arrhythmias. Native currents differ from those in experimental

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