S5395

Superoxide Dismutase from bovine erythrocytes

BioReagent, ≥3,000 units/mg protein, lyophilized powder, suitable for cell culture

• Synonyme(s) : SOD, Superoxide: superoxide oxidoreductase
• Numéro CAS: 9054-89-1
• Numéro de classification (Commission des enzymes): 1.15.1.1 (BRENDA, IUBMB)
• Numéro CE : 232-943-0
• Numéro MDL: MFCD00132404
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: bovine erythrocytes
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: lyophilized powder
• Activité spécifique: ≥3,000 units/mg protein
• Poids mol.: 32.5 kDa
• Conditionnement: pkg of 15000 units
• Technique(s): cell culture | mammalian: suitable
• pH: 7.6-10.5
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.

Application

Superoxide Dismutase (SOD) from bovine erythrocytes has been used:

• for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
• for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
• as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis

Actions biochimiques/physiologiques

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Définition de l'unité

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Remarque sur l'analyse

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)