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Key Documents

S300HR

Sigma-Aldrich

Sephacryl® Size Exclusion Resin

300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)

Synonyme(s) :

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

Numéro CAS:
65546-95-4
Numéro MDL:
MFCD00165824
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

product name

Sephacryl®, 300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)

Niveau de qualité

200

Description

stability: stable to 0.2M NaOH

Forme

suspension (20% aqueous ethanol)

Caractéristiques

autoclavable

Matrice

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

Taille des particules

25-75 μm (wet)

Dimension de pores

1-400 kDa MW range (dextrans)
10-1500 kDa MW range (globular proteins)

Compatibilité

mode of use size exclusion chromatography

Température de stockage

2-8°C

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Catégories apparentées

Description générale

S300HR-750ML′s updated product number is GE17-0599-01
  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)

Application

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Informations légales

Sephacryl is a registered trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

100.4 - 109.4 °F - closed cup

Point d'éclair (°C)

38 - 43 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

M A Soos et al.
The Biochemical journal, 263(2), 553-563 (1989-10-15)
The receptors for insulin and insulin-like growth factor-I (IGF-I) are closely related in primary sequence and overall structure. We have examined the immunological relationships between these receptors by testing the reactivity of anti-(insulin receptor) monoclonal antibodies with IGF-I receptors in
Danielle Clérot et al.
Journal of virology, 80(22), 11322-11330 (2006-09-01)
The Rep protein of tomato yellow leaf curl Sardinia virus (TYLCSV), a single-stranded DNA virus of plants, is the replication initiator essential for virus replication. TYLCSV Rep has been classified among ATPases associated with various cellular activities (AAA+ ATPases), in
Hargeet K Brar et al.
Molecular plant-microbe interactions : MPMI, 24(10), 1179-1188 (2011-06-04)
Fusarium virguliforme causes sudden death syndrome (SDS) in soybean. The pathogen has never been isolated from diseased foliar tissues; therefore, one or more toxins have been considered to cause foliar SDS development. Cell-free F. virguliforme culture filtrates containing a toxin
J M Gibbins et al.
The Journal of biological chemistry, 273(51), 34437-34443 (1998-12-16)
There is extensive evidence to show that phosphatidylinositol 3-kinase plays an important role in signaling by the immune family of receptors, which has recently been extended to include the platelet collagen receptor, glycoprotein VI. In this report we present two
Ahmet Bayrak et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(1), 49-55 (2010-12-03)
Human serum paraoxonase 1 (PON1) is a HDL-associated enzyme that catalyzes the hydrolysis of a variety of aromatic carboxylic acid esters and several organophosphates. Recently it has been suggested that a physiological substrate of serum PON1 is homocysteine thiolactone which
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