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Merck
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Key Documents

S2454

Millipore

Seppro™ Ammonium Bicarbonate Buffer

Synonyme(s) :

ABC buffer, Ammonium hydrogen carbonate, Monoammonium carbonate

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.32

Forme

solution

Niveau de qualité

200

Concentration

2 M

Technique(s)

protein expression: suitable

Couleur

clear colorless

Application(s)

life science and biopharma

Température de stockage

room temp

Description générale

Seppro® Ammonium Bicarbonate Buffer is an effective buffer for protein digestion and deglycosylation reaction. It may be used to coat 2D and 3D electrophoretic gel pieces during digestion of proteins and proteomes. The ammonium bicarbonate buffer also provides moisture during enzymatic cleavage. Ammonium bicarbonate buffer regulates the microenvironmental pH of immobilized cephalosporin C acylase (CCA) for the catalysis of cephalosporin C (CPC) in a batch stirred tank reactor (STR).

Application

Ammonium bicarbonate buffer has been used as a component of cell lysis/protein extraction buffer for proteomic sample preparation.

Informations légales

6538 is a trademark of American Type Culture Collection
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Yanhong Chang et al.
Biotechnology progress, 35(5), e2846-e2846 (2019-05-18)
In a stirred tank reactor, during catalysis with immobilized cephalosporin C acylase (CCA), the microenvironmental pH dropped to 7.2 in a nonbuffered system (with the pH maintained at 8.5 by adding alkali) due to the existence of diffusional resistance. Moreover
Emily S Boja et al.
The Journal of biological chemistry, 278(36), 34189-34202 (2003-06-12)
The zona pellucida is an extracellular matrix consisting of three glycoproteins that surrounds mammalian eggs and mediates fertilization. The primary structures of mouse ZP1, ZP2, and ZP3 have been deduced from cDNA. Each has a predicted signal peptide and a
Vimal K Balasubramanian et al.
Current protocols, 1(5), e153-e153 (2021-05-28)
Plant organs and tissues contain multiple cell types, which are well organized in 3-dimensional structure to efficiently perform physiological functions such as homeostasis and response to environmental perturbation and pathogen infection. It is critically important to perform molecular measurements at
Andrej Shevchenko et al.
Nature protocols, 1(6), 2856-2860 (2007-04-05)
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel

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