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Key Documents

RAB0817

Sigma-Aldrich

Mouse Ins1 / Insulin-1 ELISA Kit

for serum, plasma and cell culture supernatants

Synonyme(s) :

Insulin

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

mouse

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable

Entrée

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 μIU/mL
standard curve range: 6.25-400 μIU/ml

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

mouse ... Ins1(16333)

Description générale

The antibody pair provided in this kit recognizes Mouse Insulin 1.

Application

Mouse Ins1 / Insulin-1 ELISA Kit has been used to measure changes in serum insulin in male Sprague-Dawley rats with chemically induced diabetes.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Actions biochimiques/physiologiques

Certain organisms such as mice, rat and xenopus possess two non-allelic insulin genes. These genes are Ins1 and Ins2, which are both functional. They encode insulin that is produced in a 1:2 ratio, respectively in the pancreas. The two genes are differentially expressed in mouse. The Ins1 gene maps to mouse chromosome 19 in the telomeric region. The insulin that is synthesized in the pancreas is crucial in maintaining glucose homeostasis. Knockout of Ins-1 and Ins-2 leads to insulin deficient mice with diabetes mellitus, ketoacidosis, liver steatosis and retarded growth.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Description
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  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
  • RABSTOP3ELISA Stop Solution (Item I)FDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)FDS
  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)FDS
  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Differential expression and imprinting status of Ins1 and Ins2 genes in extraembryonic tissues of laboratory mice.
Deltour L, et al.
Gene expression patterns : GEP, 5(2), 297-300 (2004)
Phenotypic alterations in insulin-deficient mutant mice.
Duvillie B, et al.
Proceedings of the National Academy of Sciences of the USA, 94(10), 5137-5140 (1997)
David P Labbé et al.
Nature communications, 10(1), 4358-4358 (2019-09-27)
Systemic metabolic alterations associated with increased consumption of saturated fat and obesity are linked with increased risk of prostate cancer progression and mortality, but the molecular underpinnings of this association are poorly understood. Here, we demonstrate in a murine prostate
Snehal N Chaudhari et al.
Nature chemical biology, 17(1), 20-29 (2020-08-05)
Bariatric surgery, the most effective treatment for obesity and type 2 diabetes, is associated with increased levels of the incretin hormone glucagon-like peptide-1 (GLP-1) and changes in levels of circulating bile acids. The levels of individual bile acids in the
Evaluation of White Sesame Seed Oil on Glucose Control and Biomarkers of Hepatic, Cardiac, and Renal Functions in Male Sprague-Dawley Rats with Chemically Induced Diabetes.
Aslam F, et al.
Journal of Medicinal Food, 20(5), 448-457 (2017)
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