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RAB0252

Sigma-Aldrich

Human IL-12 p70 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type plasma
sample type urine
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.8-600 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... IL12A(3592)

Catégories apparentées

Description générale

The Human IL-12(P70) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-12(P70) in serum, plasma, cell culture supernatants and urine.

Immunogène

Recombinant Human IL-12 p70

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human IL-12 p70 ELISA kit has been used to determine the concentrations of IL-12, IL-10, IL-1β and TNFα in cell culture supernatants.

Actions biochimiques/physiologiques

IL-12A (interleukin 12 A) is essential for differentiation of Th1 (type 1) cells. Polymorphisms in IL-12A gene is associated with Graves′ and Alzheimer′s disease, rheumatoid arthritis and asthma.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)FDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)FDS
  • RABSTOP3ELISA Stop Solution (Item I)FDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Brucella Omp25 Upregulates miR-155, miR-21-5p, and miR-23b to Inhibit Interleukin-12 Production via Modulation of Programmed Death-1 Signaling in Human Monocyte/Macrophages.
Cui B, et al.
Frontiers in Immunology, 8, 708-708 (2017)
Interleukin 35 polymorphisms are associated with decreased risk of premature coronary artery disease, metabolic parameters, and IL-35 levels: the genetics of atherosclerotic disease (GEA) study.
Posadas-Sanchez R, et al.
Mediators of Inflammation, 2017, 10-10 (2017)
Jianfei Ji et al.
Stem cells translational medicine, 3(3), 356-364 (2014-01-01)
Targeting cancer stem cells (CSCs) with immunotherapy may be an effective means to prevent recurrences in glioblastoma multiforme (GBM). It is well established that CD133 is expressed in the population of GBM tumor cells representing CSCs. This raises a possibility
Xiaolu Sun et al.
BMC molecular and cell biology, 23(1), 14-14 (2022-03-21)
Pulmonary tuberculosis (TB) is a chronic infectious disease. microRNA (miR)-378 is involved in TB diagnosis. This study explored the effects of miR-378 on TB patients. A total of 126 TB patients were selected, including 63 active TB and 63 latent TB
Jin-Sung Chung et al.
The Journal of investigative dermatology, 134(11), 2784-2794 (2014-06-18)
A major barrier to successful cancer immunotherapy is the tumor's ability to induce T-cell tolerance by exploiting host regulatory mechanisms. Having discovered the DC-HIL receptor, which inhibits T-cell responses by binding to syndecan-4 on effector T cells, we posited the
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