P8045
Protein G-Biotin from Streptococcus sp.
recombinant, expressed in unspecified host, lyophilized powder
Synonyme(s) :
Biotinylated Protein G, Protein G Biotin, Protein G-Biotin
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About This Item
Produits recommandés
Produit recombinant
expressed in unspecified host
Niveau de qualité
Conjugué
biotin conjugate
Forme
lyophilized powder
Composition
Protein, ~90% biuret
Ampleur du marquage
2-4 mol d-biotin per mol protein
Technique(s)
direct ELISA: 1:70,000 using human IgG
Température de stockage
2-8°C
Description générale
Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.
Spécificité
Binds IgG only from most mammalian species except cat; binds chicken IgG.
Application
Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.
Conditionnement
Package size based on protein content
Autres remarques
Truncated - lacks albumin, Fab and membrane binding sites.
Notes préparatoires
Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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The effects of orientating pairs of synergistic monoclonal antibodies (mAb) on binding of human chorionic gonadotropin (hCG) was studied by radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). Antibody synergy towards hCG required two functionally intact antibodies
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Zacco E, et al.
Biosensors And Bioelectronics, 21(7), 1291-1301 (2006)
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