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P7130

Sigma-Aldrich

Puromycin aminonucleoside

Synonyme(s) :

3′-Amino-3′-deoxy-N6,N6-dimethyladenosine

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About This Item

Formule empirique (notation de Hill):
C12H18N6O3
Numéro CAS:
58-60-6
Poids moléculaire :
294.31
Beilstein:
93902
Numéro CE :
200-388-3
Numéro MDL:
MFCD00063462
Code UNSPSC :
51281912
ID de substance PubChem :
24898823
Nomenclature NACRES :
NA.85

Forme

powder

Niveau de qualité

300

Solubilité

H2O: soluble 50 mg/mL

Spectre d'activité de l'antibiotique

Gram-positive bacteria
neoplastics
parasites

Mode d’action

protein synthesis | interferes

Température de stockage

2-8°C

Chaîne SMILES 

CN(C)c1ncnc2n(cnc12)[C@@H]3O[C@H](CO)[C@H](N)[C@@H]3O

InChI

1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3/t6-,7+,9+,12-/m1/s1

Clé InChI

RYSMHWILUNYBFW-VENHTOENSA-N

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Catégories apparentées

Description générale

Puromycin aminonucleoside is the aminonucleoside portion of the antibiotic puromycin. It is useful in nephrology research, like studies of focal and segmental glomerulosclerosis and in the induction of nephrosis in rats. The excretion of sodium and nitrite (NOx) metabolites in rats with puromycin aminonucleoside-induced nephrotic syndrome are studied. Puromycin aminonucleoside-induced nephrosis in rats has been studied with respect to the production of reactive oxygen species during the acute phase. Puromycin aminonucleoside is used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability.

Application

Puromycin aminonucleoside has been used:
  • as a selection marker for the infected cells for the transfection of lentivirus
  • as a component of fresh medium for transfection and infection assays
  • to subcutaneously induce puromycin aminonucleoside nephrosis to investigate the mRNA and protein levels of nephrin and podocin before the onset of proteinuria

Actions biochimiques/physiologiques

Puromycin aminonucleoside is used to study human glomerular disease by inducing damage of murine glomerular podocytes and is used to study glomerular function and morphology.

Autres remarques

Keep container tightly closed in a dry and well-ventilated place.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H373

Classification des risques

STOT RE 2

Organes cibles

Kidney

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000384465
0000305755
0000305755
0000384465
108M4067V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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Ramzi Khalil et al.
The Journal of pathology, 247(2), 177-185 (2018-10-24)
Dynamin plays an essential role in maintaining the structure and function of the glomerular filtration barrier. Specifically, dynamin regulates the actin cytoskeleton and the turnover of nephrin in podocytes, and knocking down dynamin expression causes proteinuria. Moreover, promoting dynamin oligomerization
Mamunur Rashid Mahib et al.
Microbiology and immunology, 64(2), 143-152 (2019-11-07)
Inflammasomes are innate immune mechanisms that activate caspase-1 in response to a variety of stimuli, including Salmonella infection. Active caspase-1 has a potential to induce two different types of cell death, depending on the expression of the pyroptosis mediator gasdermin
Ido Refaeli et al.
Scientific reports, 10(1), 9419-9419 (2020-06-12)
Dominant and recessive mutations in podocalyxin (PODXL) are associated with human kidney disease. Interestingly, some PODXL mutations manifest as anuria while others are associated with proteinuric kidney disease. PODXL heterozygosity is associated with adult-onset kidney disease and podocalyxin shedding into
Yuqiu Lu et al.
Kidney international, 92(5), 1119-1129 (2017-07-16)
Gene expression differs substantially among individual cells of the same type. We speculate that genes that are expressed in all but a portion of cells of a given cell type would be likely essential and required for either the cell
Jeffrey A Kramer et al.
Environmental health perspectives, 112(4), 460-464 (2004-03-23)
Microarrays allow for the simultaneous measurement of changes in the levels of thousands of messenger RNAs within a single experiment. As such, the potential for the application of transcription profiling to preclinical safety assessment and mechanism-based risk assessment is profound.
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