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P6486

Millipore

EZview™ Red Protein A Affinity Gel

Synonyme(s) :

EZview Affinity Gel, Protein A Affinity Gel

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About This Item

Numéro MDL:
MFCD03456742
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.56

Forme

liquid

Classe(s) chimique(s) de l'analyte

proteins (Immunoglobulins of various mammalian species)

Technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

Capacité

~4 mg binding capacity (rabbit IgG)

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

EZview™ Red Protein A Affinity Gel is a highly visible, red colored Protein A affinity gel. The affinity resin contains Staphylococcus aureus Protein A covalently attached to crosslinked 4% agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue. The immune complexes, bound to the EZview™ Red Protein A Affinity Gel, are recovered by centrifugation. Red dye enhances visibility for more efficient results.

Application

EZview™ Red Protein A Affinity Gel has been used for purification of proteins in the preparation of immunoprecipitation and chromatin immunoprecipitation (ChIP) assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Caractéristiques et avantages

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Forme physique

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%

Stockage et stabilité

EZview™ Red Protein A Affinity Gel should be stored in 50% glycerol at -20 °C. This product is stable for at least one year when stored as indicated. Do not freeze in the absence of glycerol.

Informations légales

EZview is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H315,H317,H319,H411

Classification des risques

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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