Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

P4404

Sigma-Aldrich

Polyguanylic acid potassium salt

lyophilized powder

Synonyme(s) :

Poly(G) potassium salt

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Numéro CAS:
54684-83-2
Numéro MDL:
MFCD00131976
Code UNSPSC :
41106305
Nomenclature NACRES :
NA.51

Source biologique

enzyme from (synthesis)

Niveau de qualité

200

Pureté

≥95% (TLC)

Forme

lyophilized powder

Qualité

≤5% free nucleotides

Solubilité

water: 19.60-20.40 mg/mL, clear to slightly hazy, colorless to light yellow

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Polyguanylic acid takes up a four-stranded helical structure. It is synthetically produced in Thermus thermophilus from guanosine diphosphate (GDP) and its synthesis is catalyzed by the enzyme polynucleotide phosphorylase.

Application

Polyguanylic acid (PolyG) is used to study the effects of ionizing radiation on formation and stability of G-quadruplex structures and as a target molecule for physicochemical studies of electronic excitation (vibrational spectra) of guanosine.
Polyguanylic acid potassium salt has been used:
  • as a ligand for surface neuropilin-1 (NRP1) for internalization studies
  • for intercalation studies with trisubstituted and disubstituted triazole-linked phenyl derivatives CL41, CL42 and CL2r50 using voltammetry method
  • as a synthetic polynucleotide for agarose gel electrophoresis analysis

Actions biochimiques/physiologiques

Polyguanylic acid acts as a ligand for scavenger receptor and reduces the surface expression neuropilin-1 in endothelial cells.

Notes préparatoires

Prepared from GDP using polynucleotide phosphorylase

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Nathan R Deleault et al.
The Journal of biological chemistry, 280(29), 26873-26879 (2005-05-27)
Little is currently known about the biochemical mechanism by which induced prion protein (PrP) conformational change occurs during mammalian prion propagation. In this study, we describe the reconstitution of PrPres amplification in vitro using partially purified and synthetic components. Overnight
A study of the pH dependence of electronically excited guanosine compounds by picosecond time-resolved infrared spectroscopy.
McGovern DA, Doorley GW, et al.
Photochemistry and Photobiology, 8, 542-548 (2008)
Masashi Narazaki et al.
Blood, 116(16), 3099-3107 (2010-07-08)
Ligand interaction with cognate cell-surface receptor often promotes receptor internalization, protecting cells from prolonged or excessive signaling from extracellular ligands. Compounds that induce internalization of surface receptors prevent ligand binding to cognate cell-surface receptors serving as inhibitors. Here, we show
A Dora R Pontinha et al.
Bioelectrochemistry (Amsterdam, Netherlands), 101, 97-105 (2014-09-10)
The redox mechanism of two trisubstituted triazole-linked phenyl derivatives (CL41 and CL42) and a disubstituted triazole-linked phenyl derivative (CL2r50) were studied using cyclic, differential pulse and square wave voltammetry at a glassy carbon electrode. The CL41, CL42 and CL2r50 oxidation
Picosecond infrared probing of the vibrational spectra of transients formed upon UV excitation of stacked G-tetrad structures.
McGovern DA, Quinn S, Doorley GW, et al.
Chemical Communications (Cambridge, England), 28, 5158-5160 (2007)
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique