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P0085

Sigma-Aldrich

Anti-Podoplanin antibody, RAT monoclonal

clone NZ-1.2, purified from hybridoma cell culture

Synonyme(s) :

Anti-AGGRUS, Anti-GP36, glycoprotein, 36-KD, Anti-GP40, Anti-Gp38, Anti-OTS8, Anti-T1A, Anti-T1A-2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rat

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

NZ-1.2, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~36 kDa

Espèces réactives

human

Concentration

~1.5 mg/mL

Technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 4-8 μg/mL using U87 total cell extract

Numéro d'accès UniProt

Q86YL7

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PDPN(10630)

Catégories apparentées

Description générale

Monoclonal Anti-Podoplanin (rat IgG2a isotype) is derived from the hybridoma NZ-1.2 produced by the fusion of mouse myeloma cells and splenocytes from CD rat immunized with platelet-aggregation-stimulating (PLAG) domain of podoplanin. Podoplanin (Aggrus) is an integral membrane mucoprotein present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is a single membrane-spanning protein composed of a heavily glycosylated extracellular domain and a short intracytoplasmic tail. The extracellular domain possesses a platelet aggregation stimulating (PLAG) domain. Podoplanin has been described to be present in normal, inflammatory and neoplastic lymphatic capillary endothelium.

Immunogène

platelet-aggregation-stimulating (PLAG) domain of human podoplanin.

Application

Anti-Podoplanin antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • flow cytometry
  • immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Actions biochimiques/physiologiques

Expression of podoplanin is regulated by the lymphatic-specific homeobox gene Prox-1 (prospero-1). It is involved in lymphatic vessel formation and might be associated with tissue development and repair. Podoplanin is detected in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas. It is implicated in the onset of tumor invasion, metastasis or malignant progression. Podoplanin plays an important role in the maintenance of podocyte foot processes and glomerular permeability.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor
Kaneko MK, et al.
Febs Letters, 581(2), 331-336 (2007)
Peter Birner et al.
Cancer, 120(16), 2440-2447 (2014-04-29)
Recently, isocitrate dehydrogenase 1 (IDH1) was identified as a major participant in glioma pathogenesis. At present, the enzymatic activity of the protein has been the main topic in investigating its physiological function, but its signaling pathway allocation was unsuccessful. Interestingly
Hao Xue et al.
Autophagy, 12(7), 1129-1152 (2016-05-11)
Hypoxia induces protective autophagy in glioblastoma cells and new therapeutic avenues that target this process may improve the outcome for glioblastoma patients. Recent studies have suggested that the autophagic process is upregulated in glioblastomas in response to extensive hypoxia. Hypoxia
Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration
Ochoa-Alvarez JA, et al.
Testing, 7(7), e41845-e41845 (2012)
The potential role of podoplanin in tumour invasion
Wicki A and Christofori G
British Journal of Cancer, 96(1), 1-1 (2007)
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