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NXTRACT

Sigma-Aldrich

NuCLEAR™ Extraction Kit

For mammalian tissue or cultured cells

Synonyme(s) :

Nuclear Isolation Kit

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About This Item

Code UNSPSC :
41105517
Nomenclature NACRES :
NA.56

Niveau de qualité

300

Utilisation

 kit sufficient for 10 extractions (1 ml packed cell volume)
 kit sufficient for 100 extractions (100 μl packed cell volume)

Technique(s)

protein extraction: suitable
western blot: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.

Application

NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.

Autres remarques

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.

Liaison

Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549

Informations légales

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • 3× Dilution and Equilibration Buffer 90 mL
  • P8340Protease Inhibitor Cocktail 1 mLFDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H290,H314

Classification des risques

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Paul R Marshall et al.
Nature neuroscience, 23(6), 718-729 (2020-05-06)
DNA forms conformational states beyond the right-handed double helix; however, the functional relevance of these noncanonical structures in the brain remains unknown. Here we show that, in the prefrontal cortex of mice, the formation of one such structure, Z-DNA, is
Céline Pinheiro et al.
Journal of translational medicine, 12, 118-118 (2014-06-03)
Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current therapeutic advances, still confer a poor outcome to half of the patients. As other solid tumors, STSs exhibit high glucose consumption rates, associated with worse prognosis and therapeutic
Yu Zheng et al.
Human reproduction (Oxford, England), 27(5), 1300-1313 (2012-03-10)
Mounting evidence shows that nuclear factor-κB (NF-κB) plays an important role in endometriosis. We therefore evaluated the therapeutic potential of andrographolide, an NF-κB inhibitor. Primary cell cultures were performed using ectopic endometrial tissue specimens and their homologous eutopic endometrial specimens
Darryl L Hadsell et al.
Mammalian genome : official journal of the International Mammalian Genome Society, 29(9-10), 632-655 (2018-08-04)
The breast-feeding neonate depends on mother's milk for both macronutrients and micronutrients including minerals. The goals of the present study were to document the effects of genetic background in mice on milk concentrations of select minerals and to use genome-wide
Sumie Hiramatsu et al.
Scientific reports, 9(1), 3054-3054 (2019-03-01)
Global DNA hypomethylation in CD4+ cells in systemic lupus erythematosus (SLE) was suggested to play a key role in the pathogenesis. To identify new methylation-sensitive genes, we integrated genome-wide DNA methylation and mRNA profiling data in CD4+ cells of MRL/lpr
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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