N2787

Monoclonal Anti-Neurofilament 160 antibody produced in mouse

~2 mg/mL, clone RMO44, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Anti-Neurofilament Medium Chain
• Numéro MDL: MFCD01322393
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: RMO44, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human, mouse, rat, zebrafish
• Concentration: ~2 mg/mL
• Technique(s): immunohistochemistry: suitable; microarray: suitable; western blot: 1-2 μg/mL using extract of SHSy5y human neuroblastoma cells
• Isotype: IgG1
• Numéro d'accès UniProt: P07197
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NEFM(4741) ; mouse ... Nefm(18040) ; rat ... Nefm(24588)

Description

Description générale

Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa.

Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons.

Immunogène

purified mid-sized rat neurofilament (NF-M) subunit.

Application

Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• western blot analysis
• immunohistochemistry
• antibody labelling

Actions biochimiques/physiologiques

Neurofilament 160 (NEFM) associates with the dopamine receptor.

Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable