MTOXRTSUP

RPTEC Tox Supplement

sterile-filtered, suitable for cell culture, add to 500 ML of basal medium

• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.81

Propriétés

• Niveau de qualité: 300
• Stérilité: sterile-filtered
• Forme: liquid
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 6 mL of RPTEC Tox media supplement which is added to basal media and other components listed below to produce 500 mL of media needed for optimal culture conditions of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC) when used for Kidney Toxicology studies. The final RPTEC Tox media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).

Notes préparatoires

Prepare Tox Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-glutamine (G7513), 6 mL RPTEC Tox Supplement (MTOXRTSUP) and 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Tox Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO₂ in air atmosphere.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable