The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.
MOD50
Kit de modification de l'ADN Imprint®
For bisulfite DNA conversion & purification
Synonyme(s) :
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Description générale
The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Caractéristiques et avantages
- Only 50 picograms of DNA or 20 cells are required
- Procedure takes less than 2 hours
- Greater than 99% conversion rate
- Extremely low degradation
- Option of convenient one-step protocol
- Consistent and reproducible Bisulfite Modification
- Can be used with genomic, endonuclease digested, and FFPE DNA
Stockage et stabilité
All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.
Informations légales
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, naturalFDS
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Risques supp
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates
Peat JR, et al.
F1000Research (2017)
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Julian R Peat et al.
Methods in molecular biology (Clifton, N.J.), 1708, 161-169 (2017-12-11)
The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA
Sarah Rose Huffman et al.
Methods in molecular biology (Clifton, N.J.), 925, 201-209 (2012-08-22)
Researchers whose experimental models are mammalian oocytes and preimplantation embryos are often limited by the yield of nucleic acids that can be isolated from such a small sample size. In addition, the limited number of cells from these types of
Javed Hussain Choudhury et al.
Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that
Articles
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
Protocoles
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Contenu apparenté
The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.
-
Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?
1 answer-
Helpful?
-
-
What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?
1 answer-
DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.
Helpful?
-
-
How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?
1 answer-
This kit can be used for 50 reactions.
Helpful?
-
-
Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?
1 answer-
Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.
Helpful?
-
-
Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?
1 answer-
Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
