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MIRRT

Sigma-Aldrich

MystiCq® microRNA cDNA Synthesis Mix

Synonyme(s) :

microRNA cDNA Synthesis Mix

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.55

Technique(s)

cDNA synthesis: suitable

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Starting with total RNA or samples pre-enriched or microRNAs, the MystiCq® microRNA cDNA Synthesis Mix kit contains all the components necessary to convert mature microRNAs into cDNA templates for qPCR. Since microRNAs are not naturally polyadenylated, the first step in the cDNA synthesis process is to polyadenylate the microRNAs through a poly(A) polymerase reaction. Next, the ReadyScript reverse transcriptase, an oligo-dT adapter primer and other required reagents are added to convert the polyadenylated microRNAs into cDNA. The adapter primer includes a unique sequence at the 5′ end that is complementary to the sequence of the MystiCq Universal PCR Primer, another component of the MystiCq microRNA qPCR Assay System.The kit also contains a Positive Control Primer that is specific to SNORD44, a small nucleolar RNA that is widely expressed in most human tissues. Sufficient amounts of the Poly(A) Tailing Buffer and the MicroRNA cDNA Reaction mix are included in the kit to allow for the use of no poly(a) polymerase and no reverse transcriptase control reactions. To analyze individual microRNAs, use the MystiCq Universal PCR Primer (which targets the adapter primer sequence) together with the MystiCq microRNA qPCR Assay Primer and the MystiCq® microRNA SYBR® Green qPCR ReadyMix™.

Application

MystiCq® microRNA cDNA Synthesis Mix has been used for first-strand cDNA synthesis by reverse-transcription of miRNAs for quantitative polymerase chain reaction (qPCR) starting from total RNA

Caractéristiques et avantages

  • Separate polyadenylation and reverse transcription steps allow for the possibility of optimization of protocol if necessary
  • cDNA produced may be archived for future use
  • Single RT reaction can provide up to 1000 qPCR reactions
  • Includes a positive control primer for use with most human tissues

Conditionnement

Default reaction volume is 20 μL

Informations légales

MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Composants de kit seuls

Réf. du produit
Description
  • Poly (A) Tailing Buffer (5X)
  • Human Positive Control Primer
  • Nuclease-free Water
  • MystiCq® Universal PCR Primer
  • Poly (A) Polymerase
  • microRNA cDNA Reaction Mix
  • ReadyScript® Reverse Transcriptase

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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Dynamic changes in global microRNAome and transcriptome reveal complex miRNA-mRNA regulated host response to Japanese Encephalitis Virus in microglial cells.
Kumari B
Scientific Reports, 6, 20263-20263 (2016)
The Role of M2000 as an Anti-inflammatory Agent in Toll-Like Receptor 2/microRNA-155 Pathway.
Pourgholi F
Avicenna Journal of Medical Biotechnology, 9(1), 8-12 (2017)
Daniela Baccelli Mendonça et al.
Heliyon, 6(12), e05734-e05734 (2020-12-29)
Regulation of mTOR signaling depends on an intricate interplay of post-translational protein modification. Recently, mEAK-7 (mTOR associated protein, eak-7 homolog) was identified as a positive activator of mTOR signaling via an alternative mTOR complex. However, the upstream regulation of mEAK-7
Weerayut Wongjampa et al.
PloS one, 13(10), e0206644-e0206644 (2018-11-01)
MicroRNAs (miRNAs) are small non-coding RNAs that function to down-regulate gene expression involving in various cellular processes related to carcinogenesis. Recently, miR-22 was identified as a tumor-suppressing miRNA in many human cancers. However, the regulatory mechanism and the specific function
Mary Latimer et al.
The Journal of experimental biology, 220(Pt 16), 2932-2938 (2017-06-04)
In fish, data on microRNAs (miRNAs) involved in myogenesis are scarce. In order to identify miRNAs involved in satellite cell differentiation, we used a methionine depletion/replenishment protocol to synchronize myogenic cell differentiation. Our results validated that methionine removal (72 h) from
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Protocoles

MystiCq® MicroRNA® Quantitation System

Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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