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Key Documents

MBD0006

Sigma-Aldrich

Inactivated Akkermansia muciniphila

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/vial

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NA.51

Niveau de qualité

200

Forme

powder

Concentration

>10^8 bacteria/vial

Technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Description générale

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Inactivated Akkermansia muciniphila can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Akkermansia muciniphila is a gram negative, anaerobic, non motile, non spore forming and oval shaped bacterium. A. muciniphila inhabits the gastrointestinal tracts of more than 90% of adults and constitutes 1 to 4% of the fecal microbiota.2 A. muciniphila level was suggested to be a biomarker for a healthy intestin. 3
A. muciniphila degrades mucus and utilizes it as a carbon/nitrogen source. Consequently, the host produces additional mucus while the bacterium produces oligosaccharides and Short Chain Fatty Acids (SCFAs) that can be utilized by the host and trigger the immune system. An additional protective effect of the SCFA is stimulation of mucus-associated microbiota growth, that serves as a barrier against penetration of pathogens to intestinal cells. 2,4

Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Inactivated bacteria are provided in at >10^8 bacteria/ml concentration in TE buffer pH 8.0.It is recommended to avoid freeze thaw cycles of this product.

Forme physique

Liquid -The inactivated bacteria is provided at >10^8 bacteria/ml in TE buffer pH 8.0

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

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