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MAK451

Sigma-Aldrich

Glucose-6-Phosphate Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

Synonyme(s) :

G6PD Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Niveau de qualité

100

Entrée

plasma
serum
tissue
cell culture medium

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

hematological disorder, anemia

Température de stockage

−20°C

Description générale

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.

Application

The Glucose-6-Phosphate Dehydrogenase Assay Kit may be used for:
  • Metabolism Research
  • Drug Discovery

Caractéristiques et avantages

  • Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose-6-phosphate dehydrogenase levels across a wide range from 0.2 to 100 U/L, offering both convenience and sensitivity.
  • Compatibility with High-Throughput Systems: Effortlessly incorporate our kit into high-throughput handling systems, guaranteeing seamless integration and maintaining efficiency and accuracy in your laboratory operations.

Adéquation

The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.

Principe

The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H341

Classification des risques

Muta. 2

Code de la classe de stockage

12 - Non Combustible Liquids

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