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Key Documents

MAK126

Sigma-Aldrich

Bilirubin Assay Kit

sufficient for 180 colorimetric tests

Synonyme(s) :

Hematoidin Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 180 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

hematological disorder; gastrointestinal diseases

Température de stockage

2-8°C

Description générale

Bilirubin, also known as hematoidin, is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease, blood disorders, or blockage of the bile ducts.

Application

Bilirubin Assay Kit has been used to measure the total bilirubin concentration in samples.

Caractéristiques et avantages

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Adéquation

Suitable for the detection of of both total and conjugated bilirubin in serum and plasma samples.

Principe

This assay, based on the Jendrassik-Grof method, utilizes the reaction of bilirubin with diazotized sulfanilic acid resulting in a colorimetric product measured at 530 nm, proportionate to the bilirubin present in the sample. This assay kit measures both total and conjugated bilirubin.

Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Morgane M Thibaut et al.
Journal of cachexia, sarcopenia and muscle, 12(1), 70-90 (2020-12-23)
Cancer cachexia is a debilitating metabolic syndrome contributing to cancer death. Organs other than the muscle may contribute to the pathogenesis of cancer cachexia. This work explores new mechanisms underlying hepatic alterations in cancer cachexia. We used transcriptomics to reveal
Ji-Yoon Kim et al.
BMB reports, 53(3), 148-153 (2019-08-14)
Erythropoietin and iron have individually shown beneficial effects on early-phase liver regeneration following partial hepatectomy (PHx); however, there are limited data on the combined effect on late-phase liver regeneration after PHx. Here we examined combined effects of recombinant human erythropoietin
Bin Dong et al.
Journal of lipid research, 58(2), 350-363 (2016-12-13)
The farnesoid X receptor (FXR) plays critical roles in plasma cholesterol metabolism, in particular HDL-cholesterol (HDL-C) homeostasis. Obeticholic acid (OCA) is a FXR agonist being developed for treating various chronic liver diseases. Previous studies reported inconsistent effects of OCA on
Zhi-Ming Ding et al.
Frontiers in pharmacology, 9, 410-410 (2018-05-17)
Understanding of the temporal changes of hepatic lesions in the progression and regression of non-alcoholic steatohepatitis (NASH) is vital to elucidation of the pathogenesis of NASH, and critical to the development of a strategy for NASH pharmacotherapy. There are challenges
Progression and regression of hepatic lesions in a mouse model of NASH induced by dietary intervention and its implications in pharmacotherapy.
Ding ZM, et al.
Frontiers in Pharmacology, 9, 410-410 (2018)

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