MAK114
Glyoxalase I Activity Assay Kit
sufficient for 100 tests (UV)
Synonyme(s) :
Aldoketomutase Activity Assay, GLO1 Activity Assay, Ketone-Aldehyde Mutase, Activity Assa, Lactoylglutathione Lyase Activity Assay, Methylglyoxalase Activity Assay
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 100 tests (UV)
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
cancer
Température de stockage
−20°C
Informations sur le gène
human ... GLO1(2739)
mouse ... GLO1(109801)
rat ... GLO1(294320)
Description générale
Glyoxalase I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methyglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans, and cancer.
Application
Glyoxalase I Assay Kit has been used to estimate the glyoxalase I activity in plasma samples.
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
Suitable for the detection of Glyoxalase I activity in enzyme preparations and biological samples. Suitable for testing the effects of compounds on Glyoxalase I activity.
Principe
The Glyoxalase I Activity Assay kit provides a simple and direct procedure for measuring GLO-1 activity in a variety of samples such as enzyme preparations or biological samples. In this assay, the GLO-1-mediated production of S-lactoylglutathione is measured by monitoring the change in absorbance at 240 nm. One unit of Glyoxalase I is the amount of enzyme that will convert 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.6 and 25 °C.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
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Naimesh Solanki et al.
The international journal of neuropsychopharmacology, 20(7), 550-561 (2017-03-25)
Persistent psychological stress often leads to anxiety disorders and depression. Benzodiazepines and selective serotonin reuptake inhibitors are popular treatment options but have limited efficacy, supporting the need for alternative treatment. Based on our recent preclinical work suggesting a causal link
Mandy C Szymanski et al.
Journal of applied physiology (Bethesda, Md. : 1985), 124(2), 330-340 (2017-09-25)
Szymanski MC, Gillum TL, Gould LM, Morin DS, Kuennen MR. Short-term dietary curcumin supplementation reduces gastrointestinal barrier damage and physiological strain responses during exertional heat stress. J Appl Physiol 124: 330-340, 2018. First published September 21, 2017; doi: 10.1152/japplphysiol.00515.2017 .-This
Modulating oxidative stress relieves stress-induced behavioral and cognitive impairments in rats.
Solanki N, et al.
The International Journal of Neuropsychopharmacology, 20(7), 550-561 (2017)
Tiago Rodrigues et al.
Pharmacological research, 161, 105198-105198 (2020-09-18)
Methylglyoxal was shown to impair adipose tissue capillarization and insulin sensitivity in obese models. We hypothesized that glyoxalase-1 (GLO-1) activity could be diminished in the adipose tissue of type 2 diabetic obese patients. Moreover, we assessed whether such activity could
Jin-Quan Huang et al.
Nature chemical biology, 16(3), 250-256 (2020-01-15)
In plants, lineage-specific metabolites can be created by activities derived from the catalytic promiscuity of ancestral proteins, although examples of recruiting detoxification systems to biosynthetic pathways are scarce. The ubiquitous glyoxalase (GLX) system scavenges the cytotoxic methylglyoxal, in which GLXI
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