Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

View All Documentation

MAK107

Sigma-Aldrich

LCAT Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Synonyme(s) :

Lecithin:cholesterol acyltransferase activity assay kit

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Numéro CE :
200-659-6
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 fluorometric tests

Application(s)

pharmaceutical

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

cardiovascular diseases

Température de stockage

−20°C

Informations sur le gène

human ... LCAT(3931)

Description générale

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

Application

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.
LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for measuring phospholipase activity of LCAT in plasma or serum.

Principe

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λEx=390 nm/λEm=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

Remarque sur l'analyse

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Composants de kit seuls

Réf. du produit
Description
  • Substrate Reagent .1 mL
  • READ Reagent 30 mL
  • LCAT Assay Buffer 20 mL

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

FlameSkull and crossbonesHealth hazard
GHS02,GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

49.5 °F

Point d'éclair (°C)

9.7 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 1

1 of 1

Streptozocine ≥75% α-anomer basis, ≥98% (HPLC), powder

Sigma-Aldrich

S0130

Streptozocine

Ameliorative potential of gingerol: Promising modulation of inflammatory factors and lipid marker enzymes expressions in HFD induced obesity in rats.
Naidu P B, et al.
Molecular and Cellular Endocrinology, 419, 139-147 (2016)
Reversal of high fat diet-induced obesity through modulating lipid metabolic enzymes and inflammatory markers expressions in rats.
Uddandrao V V S, et al.
Archives of Physiology and Biochemistry, 1-7 (2018)
Akbar Jafarnejad et al.
The Journal of pharmacology and experimental therapeutics, 324(2), 850-857 (2007-11-15)
Diabetes mellitus has been classified as a conformational disease because of changes induced in the structure and function of proteins due to hyperglycemia. In this study, we investigated the effect of high-dose and long-term use of acetyl salicylic acid (ASA)
Anatol Kontush et al.
Arteriosclerosis, thrombosis, and vascular biology, 27(8), 1843-1849 (2007-06-16)
The purpose of this study was to define heterogeneity in the molecular profile of lipids, including sphingomyelin and sphingosine-1-phosphate, among physicochemically-defined HDL subpopulations and potential relevance to antiatherogenic biological activities of dense HDL3. The molecular profile of lipids (cholesteryl esters
Mitigating efficacy of piperine in the physiological derangements of high fat diet induced obesity in Sprague Dawley rats.
BrahmaNaidu P, et al.
Chemico-Biological Interactions, 221, 42-51 (2014)
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique