MAK059
Formate Assay Kit
sufficient for 100 colorimetric tests
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 100 colorimetric tests
Application(s)
cosmetics
environmental
food and beverages
Méthode de détection
colorimetric
Température de stockage
−20°C
Description générale
Formate, the salt form of formic acid, is the simplest carboxylate anion. Formate is produced in the mitochondria as a result of the oxidation of one-carbon donors such as serine. Mitochondrially-derived formate is utilized for cytoplasmic one-carbon metabolism in the de novo synthesis of purines, thymdiylate, and the remethylation of homocysteine to methionine. Under normal physiologic conditions, formate is present at low levels in serum and blood but levels may be increased following acute methanol poisoning or environmental exposure to formaldehyde. Serum and urine formate levels were increased in vitamin B12-deficient rats and may be indicative of hyperhomocysteinemia due to defects in remethylation.
Application
Formate Assay kit has been used to determine the concentration of formate in samples.
Adéquation
Suitable for serum, urine, media, cells, and tissues.
Principe
The Formate Assay Kit provides a simple, sensitive, and rapid means of quantifying formate in a variety of samples including serum, urine, media, cells, and tissues. Formate concentrations are determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the formate present.
Autres remarques
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Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
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Providing reducing power by microalgal photosynthesis: a novel perspective towards sustainable biocatalytic production of bulk chemicals exemplified for aliphatic amines
Lowe J, et al.
Scientific Reports, 8, 10436-10436 (2018)
Simon G Lamarre et al.
American journal of physiology. Endocrinology and metabolism, 302(1), E61-E67 (2011-09-22)
Formate can differentiate between hyperhomocysteinemia due to impaired remethylation and impaired transsulfuration. Am J Physiol Endocrinol Metab 301: E000-E000, 2011. First published September 20, 2011; 10.1152/ajpendo.00345.2011.-We carried out a (1)H-NMR metabolomic analysis of sera from vitamin B(12)-deficient rats. In addition
Benjamin J Koestler et al.
mBio, 9(5) (2018-09-27)
The intracellular human pathogen Shigella flexneri invades the colon epithelium, replicates to high cell density within the host cell, and then spreads to adjacent epithelial cells. When S. flexneri gains access to the host cytosol, the bacteria metabolize host cytosolic
Henning Kirst et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(8) (2022-02-24)
Formate has great potential to function as a feedstock for biorefineries because it can be sustainably produced by a variety of processes that don't compete with agricultural production. However, naturally formatotrophic organisms are unsuitable for large-scale cultivation, difficult to engineer
Jana Löwe et al.
Scientific reports, 8(1), 10436-10436 (2018-07-12)
A biotechnological process is reported, which enables an enzymatic reduction without the need for addition of an organic co-substrate for in situ-cofactor recycling. The process is based on merging the fields of enzymatic reductive amination with formate dehydrogenase-based in situ-cofactor
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