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Merck
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Key Documents

MAK048

Sigma-Aldrich

Lipase Activity Assay Kit III

sufficient for 100 fluorometric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 fluorometric tests

Méthode de détection

fluorometric

Température de stockage

−20°C

Description générale

The lipase family of enzymes catalyzes the cleavage of the ester bonds of lipids. In mammals, this family includes many critical members including pancreatic, hepatic, endothelial, and lipoprotein lipase. Lipases, such as pancreatic lipase, are critical for the metabolism of lipids. Lipases also play key roles in processes such as cell signaling and inflammation. Measurements of lipase activity are commonly used to screen for pancreatic injury or disease, and to monitor diseases such as cystic fibrosis, celiac disease, and Crohn′s disease.

Application


  • High-Temperature Short-Time and Holder Pasteurization of Donor Milk: Impact on Milk Composition.: This study evaluates the effects of different pasteurization methods on the composition of donor milk, focusing on its nutritional and biochemical properties (Escuder-Vieco et al., 2021).

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for the detection of lipase activity in biological samples including tissue, cells and serum

Principe

The Lipase Activity Assay Kit III provides a simple and direct procedure for measuring lipase activity in a variety of samples. Lipase activity is determined using a coupled enzyme reaction, which results in the generation of methylresorufin (λex = 529/λem = 600 nm) proportional to the enzymatic activity present. One unit of lipase is the amount of enzyme that will generate 1.0 μmole of methylresorufin from the substrate per minute at 37 °C.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids


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