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M8787

Sigma-Aldrich

Chlorure de magnésium solution

PCR Reagent, 25 mM MgCI2 solution for PCR

Synonyme(s) :

PCR optimization

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About This Item

Formule linéaire :
MgCl2
Numéro CAS:
7786-30-3
Poids moléculaire :
95.21
Numéro MDL:
MFCD00011106
Code UNSPSC :
12352302
ID de substance PubChem :
57654422
Nomenclature NACRES :
NA.52

Qualité

PCR Reagent

Niveau de qualité

200
300

Forme

liquid

Conditionnement

vial of 1.5 mL

Concentration

25 mM±1 mM

Technique(s)

PCR: suitable

Couleur

colorless

Application(s)

agriculture
diagnostic assay manufacturing

Activité étrangère

DNase, RNase, none detected

Température de stockage

2-8°C

Chaîne SMILES 

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

Clé InChI

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Description générale

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Application

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression

Adéquation

Suitable for optimization of polymerase chain reactions.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Molecular characterization of Fusarium oxysporum f. sp. cubense isolates from banana
Das A, et al.
Pest Management in Horticultural Ecosystems , 18(2), 171-178 (2012)
Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
Adewumi G A, et al.
Food Biotechnology, 28(3), 250-268 (2014)
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
Benjamin Dombert et al.
PloS one, 9(10), e110846-e110846 (2014-10-23)
Spinal muscular atrophy (SMA) is caused by deficiency of the ubiquitously expressed survival motoneuron (SMN) protein. SMN is crucial component of a complex for the assembly of spliceosomal small nuclear ribonucleoprotein (snRNP) particles. Other cellular functions of SMN are less
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Multiplex PCR Assay for Damaged DNA

Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

Multiplex PCR Assay for Damaged DNA

Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

Multiplex PCR Assay for Damaged DNA

Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

Multiplex PCR Assay for Damaged DNA

Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

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Protocoles

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

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