M6667

Monocyte Chemotactic Protein-1 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : MCP-1
• Numéro MDL: MFCD00131621
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Pureté: ≥97% (SDS-PAGE and N-terminal analysis)
• Forme: lyophilized powder
• Puissance: 5-60 ng/mL EC₅₀
• Poids mol.: 8.7 kDa
• Conditionnement: pkg of 10 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P13500
• Température de stockage: −20°C
• Informations sur le gène: human ... CCL2(6347)

Description

Description générale

Monocyte chemoattractant protein-1 (MCP-1) or monocyte chemotactic and activating factor (MCAF) is part of the C-C chemokine family. The gene encoding this protein is localized on human chromosome 17q12.

Application

Monocyte chemotactic protein-1 (MCP-1) has been used to study its effects on the motility of lymphoblastoid cell line NC–NC cells.

Actions biochimiques/physiologiques

The biological activity is measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A. The ED<SUB>50</SUB> for this effect is typically 5-30 ng/mL.

Measured by its ability to chemoattract 2-day cultured human monocytes, Matsushima, K., et al. (1989) J. Exp. Med. 169:1485, the ED<SUB>50</SUB> for this effect is typically 5-20 ng/mL.

Monocyte chemoattractant protein-1 (MCP-1) triggers the activation of monocytes by triggering degranulation as well as respiratory burst activity in monocytes. It facilitates monocytes in inhibiting or enhancing the growth of specific brain tumor cell lines. MCP-1 chemoattracts monocytes, macrophages, T cells and natural killer cells. The protein is overexpressed in atherosclerosis, idiopathic pulmonary fibrosis, rheumatoid arthritis and delayed hypersensitivity reactions. It is also implicated in Kawasaki disease.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)