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Key Documents

M5774

Sigma-Aldrich

Anti-Mouse Serum antibody produced in rabbit

whole antiserum

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

whole antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Contient

15 mM sodium azide

Espèces réactives

mouse

Technique(s)

immunoelectrophoresis: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Mouse serum comprises hormones, antigens, electrolytes and antibodies. It serves blocking agent in immunoassays.

Spécificité

Strong reactivity with normal mouse serum has been determined by immunoelectrophoresis (IEP). This antiserum has not been assayed for interspecies crossreactivity.

Application

Anti-Mouse Serum antibody produced in rabbit has been used in differential fluorescent labelling. It has also been used in differential staining of inner cell mass (ICM) and and trophectoderm (TE) cells. It has been also been used in the removal of zona pellucidae for the isolation of inner cell mass (ICM) from mouse blastocysts.
Anti-Mouse Serum antibody produced in rabbit was used as blocking agent in differential cell count of trophectoderm and inner cell mass cells of mouse embryos.

Forme physique

Rabbit Anti-Mouse Serum is provided as a liquid containing 15 mM sodium azide as preservative.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Carla Mulas et al.
Development (Cambridge, England), 146(6) (2019-03-28)
The power of mouse embryonic stem (ES) cells to colonise the developing embryo has revolutionised mammalian developmental genetics and stem cell research. This power is vulnerable, however, to the cell culture environment, deficiencies in which can lead to cellular heterogeneity
Raúl Fernández-González et al.
Reproduction (Cambridge, England), 137(2), 271-283 (2008-11-20)
We have reported that in vitro culture (IVC) of preimplantation mouse embryos in the presence of FCS produces long-term effects (LTE) on development, growth and behaviour of the offspring at adult age. To analyse the mechanisms underlying this phenomenon, we
Bi-Ke Zhu et al.
Reproduction, nutrition, development, 44(6), 617-629 (2005-03-15)
The objective of this study was to examine the effect of paternal heat stress on the in vivo development of preimplantation embryos in the mouse. Synchronised B6CBF1 female mice were mated either to a control male mouse or to one
Both the folate cycle and betaine-homocysteine methyltransferase contribute methyl groups for DNA methylation in mouse blastocysts
Zhang B, et al.
Faseb Journal, 29(3), 1069-1079 (2015)
J Van der Elst et al.
Human reproduction (Oxford, England), 13(6), 1595-1599 (1998-08-04)
We demonstrated previously that ultra-rapid freezing of mouse oocytes with 3.5 M dimethylsulphoxide (DMSO) decreased cell numbers in day 5 in-vitro cultured blastocysts. In the present study we counted cell numbers of trophectoderm (TE) and inner cell mass (ICM) separately

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