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Key Documents

M1533

Sigma-Aldrich

N,N′-Methylenebisacrylamide solution

suitable for electrophoresis, 2% in H2O

Synonyme(s) :

BIS

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About This Item

Formule linéaire :
(CH2=CHCONH)2CH2
Numéro CAS:
Poids moléculaire :
154.17
Numéro Beilstein :
1706297
Numéro MDL:
Code UNSPSC :
41105319
ID de substance PubChem :
Nomenclature NACRES :
NB.22

Stérilité

0.2 μm filtered

Niveau de qualité

Forme

liquid

Concentration

2% in H2O

Technique(s)

electrophoresis: suitable

Température de stockage

2-8°C

Chaîne SMILES 

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

Clé InChI

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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Description générale

N,N′-Methylenebisacrylamide solution is a cross-linking agent used in polyacrylamide gel preparation.

Application

N,N′-Methylenebisacrylamide solution has been used as a component of hydrogel precursor solution and magnified analysis of the proteome (MAP) gel solution during hydrogel fabrication. It has also been used as a component of re-embedding solution during re-embedding of expanded hydrogels.
To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Notes préparatoires

Prepared from electrophoresis grade bis-acrylamide in ultra-pure water.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Carc. 1B - Muta. 1B - STOT RE 2 Oral

Organes cibles

Peripheral nervous system

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)


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Chapter 15 - Techniques for Protein Analysis
Barh D and Azevedo V
Omics : A Journal of Integrative Biology null
A J Aho et al.
British journal of anaesthesia, 115(2), 258-266 (2015-07-04)
Concomitantly recorded Bispectral Index® (BIS) and Entropy™ values sometimes show discordant trends during general anaesthesia. Previously, no attempt had been made to discover which EEG characteristics cause discrepancies between BIS and Entropy. We compared BIS and Entropy values, and analysed
Alice E Stanton et al.
Acta biomaterialia, 96, 310-320 (2019-07-01)
Extracellular matrix (ECM) is comprised of different types of proteins, which change in composition and ratios during morphogenesis and disease progression. ECM proteins provide cell adhesion and impart mechanical cues to the cells. Increasing substrate stiffness has been shown to
Jean Guglielminotti et al.
Anesthesiology, 122(5), 985-993 (2015-03-03)
Individual assessment of the amplitude of a physiologic reflex evoked by a standardized noxious test (SNT) before surgical stimulation has been suggested to predict movement upon the forthcoming surgical stimulation. This study aimed to compare the ability of pupillary dilatation
David R Kryscio et al.
Analytica chimica acta, 718, 109-115 (2012-02-07)
Molecularly imprinted polymers are synthetic antibody mimics formed by the crosslinking of organic or inorganic polymers in the presence of an analyte which yields recognitive polymer networks with specific binding pockets for that biomolecule. Surface imprinted polymers were synthesized via

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