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Key Documents

M0894

Sigma-Aldrich

Milieu essentiel minimum d′Eagle

Alpha Modification, with L-glutamine and sodium pyruvate, without ribonucleosides, deoxyribonucleosides and sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

MEM

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Niveau de qualité

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Composants

stable glutamine: no
Hanks’ salts (2% CO2): no
sodium pyruvate: yes
NaHCO3: no
phenol red: yes
glucose: yes
L-glutamine: yes
Earle’s salts (5% CO2): yes
HEPES: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Quantité

Formulated to contain 10.1 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Alexander J Nelson et al.
Journal of lipid research, 61(2), 143-158 (2019-12-11)
Phospholipases A2 (PLA2s) catalyze hydrolysis of the sn-2 substituent from glycerophospholipids to yield a free fatty acid (i.e., arachidonic acid), which can be metabolized to pro- or anti-inflammatory eicosanoids. Macrophages modulate inflammatory responses and are affected by Ca2+-independent phospholipase A2
Jason W Ashley et al.
The Journal of biological chemistry, 291(44), 23268-23281 (2016-10-30)
Macrophages are important in innate and adaptive immunity. Macrophage participation in inflammation or tissue repair is directed by various extracellular signals and mediated by multiple intracellular pathways. Activation of group VIA phospholipase A2 (iPLA2β) causes accumulation of arachidonic acid, lysophospholipids
Tatsuki Kawasaki et al.
Development, growth & differentiation, 58(6), 530-545 (2016-06-01)
The periodontal ligament (PDL) is a connective tissue that attaches the tooth cementum to the alveolar bone and is derived from dental follicle cells (DFCs). The DFCs form fibroblasts, osteoblasts, cementoblasts, and PDL stem cells (PDLSCs). We previously reported homeobox
Yuxuan Xiao et al.
Biochemical and biophysical research communications, 487(3), 640-645 (2017-04-25)
The meiotic G2/M1 transition is mostly regulated by posttranslational modifications, however, the cross-talk between different posttranslational modifications is not well-understood, especially in spermatocytes. Sumoylation has emerged as a critical regulatory event in several developmental processes, including reproduction. In mouse oocytes
Kateřina Valentová et al.
International journal of molecular sciences, 19(8) (2018-08-12)
Silymarin, an extract from milk thistle (Silybum marianum) fruits, is consumed in various food supplements. The metabolism of silymarin flavonolignans in mammals is complex, the exact structure of their metabolites still remains partly unclear and standards are not commercially available.

Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocoles

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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