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Key Documents

M0404

Sigma-Aldrich

Milieu de base de Murashige et Skoog

powder, suitable for plant cell culture, with Gamborg′s vitamins

Synonyme(s) :

Milieu de base MS

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.72

Niveau de qualité

Forme

powder

Technique(s)

cell culture | plant: suitable

Application(s)

agriculture

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Application

Murashige and Skoog Basal Medium has been used:
  • to germinate the seeds for seedling growth and germination studies
  • in the suspension culture of Nicotiana plumbaginifolia for transformation
  • in the root culture of hairy clone A. artemisiifolia T4

Variante de formule

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantité

Formulated to contain 4.4 grams of powder per liter of medium.

Notes préparatoires

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Pictogrammes

Flame over circleExclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2 - Ox. Sol. 3

Code de la classe de stockage

5.1B - Oxidizing hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Itai Bloch et al.
Plant biotechnology journal, 19(9), 1785-1797 (2021-03-28)
The synthesis of essential amino acids in plants is pivotal for their viability and growth, and these cellular pathways are therefore targeted for the discovery of new molecules for weed control. Herein, we describe the discovery and design of small
Statistical analysis of elicitation strategies for thiarubrine A production in hairy root cultures of Ambrosia artemisiifolia
Bhagwath SG and Hjortso MA
Journal of Biotechnology, 80(2), 159-167 (2000)
John E McLaughlin et al.
PloS one, 10(6), e0130204-e0130204 (2015-06-10)
Fusarium head blight (FHB) or scab is one of the most important plant diseases worldwide, affecting wheat, barley and other small grains. Trichothecene mycotoxins such as deoxynivalenol (DON) accumulate in the grain, presenting a food safety risk and health hazard
Brenden Hurley et al.
PloS one, 9(12), e114921-e114921 (2014-12-17)
Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity through Its ADP-ribosyltransferase activity. Proteomic analysis of HopF2 interacting proteins identified a protein complex containing ATPases
Crescencio Bazaldúa et al.
PloS one, 14(9), e0222464-e0222464 (2019-09-13)
Ten Hyptis suaveolens hairy root lines were established by infecting nodal explants with K599+pGus-GFP+ and ATCC15834+pTDT strains from Agrobacterium rhizogenes. Genetic transformation was confirmed by epifluorescence and plagiotropic hairy root growth in absence of growth regulators. Cytotoxicity was determined using

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