When reconstituted and filter sterilized, this medium is expected to have a similar shelf-life as prepared 1X Medium 199 with Hanks salts and L-glutamine. See product 51322C for the prepared liquid medium. This product has an expiration date of 1 year from the date of quality release. Upon the addition of serum or other heat-sensitive components, the shelf life would be limited to 1 - 2 weeks at 2 - 8°C. Freeze/thaw cycles may result in precipitate formation.
M0393
Medium 199
With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
M199 Medium, TCM 199
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Nom du produit
Medium 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Composants
HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to test and determine the pH sensitivity of the sensors in a complex environment
- as a culture medium during oocyte harvesting
- as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani
- to culture visceral adipose tissue explants
Quantité
Formulated to contain 10.6 grams of powder per liter of medium.
Reconstitution
Supplement with 0.35 g/L sodium bicarbonate.
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
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Johannes Dittmann et al.
Journal of the mechanical behavior of biomedical materials, 77, 764-775 (2017-08-12)
The present study focused on the material characterisation of porcine oocytes by experiments combined with an inverse finite-element method (iFEM) approach. In doing so, two different deformation states, compression and indentation, were realised to enable the validation of the numerical
Alberto Stracuzzi et al.
Biomechanics and modeling in mechanobiology, 20(2), 751-765 (2021-02-04)
Probing mechanical properties of cells has been identified as a means to infer information on their current state, e.g. with respect to diseases or differentiation. Oocytes have gained particular interest, since mechanical parameters are considered potential indicators of the success
Wen-Wei Zhang et al.
BMC infectious diseases, 18(1), 500-500 (2018-10-05)
Visceral leishmaniasis (VL), caused by Leishmania donovani complex parasites, is a neglected parasitic disease that is generally fatal if untreated. Despite decades of research to develop a sensitive VL diagnostic test, definitive diagnosis of VL still mainly relies on the
Articles
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Protocoles
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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Once prepared, how long can the media stay refrigerated? Can it be frozen?
1 answer-
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