L7914

Lipoprotein, low density from human plasma

≥95% (SDS-PAGE), solution

• Synonyme(s) : β-Lipoprotein, LDL, Low density lipoprotein
• Numéro CAS: 308068-14-6
• Numéro MDL: MFCD00131524
• Code UNSPSC : 12352211
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: human plasma
• Niveau de qualité: 200
• Pureté: ≥95% (SDS-PAGE)
• Forme: solution
• Numéro d'accès UniProt: P02649; P02655; P04114
• Groupe fonctionnel: ester; phospholipid
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Informations sur le gène: human ... APOB(338) , APOC2(344) , APOE(348)

Description

Application

Lipoprotein, low density from human plasma has been used:

• as an additive in cholesterol-free RPMI media
• as a component in buffer to perform an assay for measuring lipid peroxidation
• to stimulate hepatic macrophages
• as a plasma protein to determine pazopanib unbound fraction (fu%) by equilibrium dialysis

Actions biochimiques/physiologiques

LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits; receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle resulting dissociation of LDL from its receptor. After loss of the clathrin coat the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake.

Attention

Freezing of lipoprotein solutions may cause structural or compositional changes.

Propriétés physiques

Low density lipoproteins are smaller than VLDL and IDL (26 nm) (MW approximately 3.5 million) and more dense (~1.04). The protein component of LDL is apolipoprotein B100. LDL contains 20-22% protein, 10-15% triglycerides, 20-28% phospholipids, 37-48% cholesteryl esters and 8-10% cholesterol.

Forme physique

Solution in 150 mM NaCl and 0.01% EDTA, pH 7.4

Autres remarques

View more information on lipoprotein function and lipid transport at www.sigma-aldrich.com/enzymeexplorer

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable