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L7293

Sigma-Aldrich

Monoclonal Anti-LSD1 (AOF2) antibody produced in mouse

~2 mg/mL, clone LSD1-12, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Anti-Amine oxidase, flavin-containing, 2, Anti-BHC110, Anti-BRAF-HDAC complex protein 110, Anti-KIAA0601 protein, Anti-Lysine-specific demethylase 1

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

LSD1-12, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~110 kDa

Espèces réactives

mouse, human

Concentration

~2 mg/mL

Comparer avec des articles similaires

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Cet article
36749450032K6250
assay

≥98% (GC)

assay

99%

assay

≥97.0% (KT)

assay

≥99% (TLC)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

solubility

50 mg/mL, clear, colorless to faintly yellow (1 N Hydrochloric acid)

solubility

-

solubility

-

solubility

water: 49-51 mg/mL

form

crystalline

form

powder or crystals

form

solid

form

powder

color

beige

color

-

color

white

color

-

Description générale

Monoclonal Anti-LSD1 (AOF2) (mouse IgG1 isotype) is derived from the hybridoma LSD1-12 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Lysine specific demethylase 1 (LSD1) also known as amine oxidase flavin-containing domain 2 (AOF2) () is a lysine-specific demethylase. It is a nuclear protein containing a SWIRM (Swi3p, Rsc8p and Moira) domain, a flavin adenine dinucleotide (FAD)-binding motif and an amine oxidase domain. The LSD1 gene is mapped to human chromosome 1p36.12. LSD1 is referred to by additional names including KDM1A protein, BHC110(BRAF-HDAC complex protein 110) and nuclear polyamine oxidase (NPAO)().

Spécificité

Monoclonal Anti-LSD1 (AOF2) recognizes human and mouse LSD1.

Immunogène

synthetic peptide corresponding to amino acids 832-847 of human LSD1/BHC110. This sequence is identical in bovine, rat, mouse, and frog.

Application

Monoclonal Anti-LSD1 (AOF2) antibody produced in mouse has been used:
  • immunofluorescence
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

Actions biochimiques/physiologiques

Lysine Specific Demethylase 1 (LSD1) is a component of several histone deacetylase complexes that function through modifying chromatin structure to repress transcription.LSD1 action can signal either activation or repression of transcription. Demethylation of histone H3 Lys4 by LSD1 leads to transcriptional repression of target genes, while demethylation of histone H3 at Lys9 leads to depression of androgen receptor target genes. The activity of LSD1 (AOF2) is regulated by its associated factors namely, corepressor to the REST (RE1 silencing transcription factor/neural restrictive silencing factor)(CoREST). This, in turn, promotes demethylation by enhancing the association of LSD1 with the nucleosomes and also protects LSD1 from proteasomal degradation. LSD1 is a component of nucleosome remodeling and deacetylase (NuRD). It regulates neuronal development and favors neural stem cell proliferation. It highly expressed in acute myeloid leukemia and many solid tumors.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The cancer driver genes IDH1 2, JARID1C KDM5C, and UTX KDM6A: crosstalk between histone demethylation and hypoxic reprogramming in cancer metabolism
Chang S, et al.
Experimental & Molecular Medicine, 51(6), 1-17 (2019)
Jing Wang et al.
Nature genetics, 41(1), 125-129 (2008-12-23)
Histone methylation and DNA methylation cooperatively regulate chromatin structure and gene activity. How these two systems coordinate with each other remains unclear. Here we study the biological function of lysine-specific demethylase 1 (LSD1, also known as KDM1 and AOF2), which
Daniela Magliulo et al.
Frontiers in oncology, 8, 255-255 (2018-08-04)
Acute myeloid leukemia (AML) is a heterogeneous hematopoietic malignancy characterized by the accumulation of incompletely differentiated progenitor cells (blasts) in the bone marrow and blood, and by suppression of normal hematopoiesis. It has recently become apparent that the AML genome
Alba Maiques-Diaz et al.
Epigenomics, 8(8), 1103-1116 (2016-08-02)
LSD1 (KDM1A; BHC110; AOF2) was the first protein reported to exhibit histone demethylase activity and has since been shown to have multiple essential roles in mammalian biology. Given its enzymatic activity and its high-level expression in many human malignancies, a
Yu-Jiang Shi et al.
Molecular cell, 19(6), 857-864 (2005-09-06)
LSD1 is a recently identified human lysine (K)-specific histone demethylase. LSD1 is associated with HDAC1/2; CoREST, a SANT domain-containing corepressor; and BHC80, a PHD domain-containing protein, among others. We show that CoREST endows LSD1 with the ability to demethylate nucleosomal

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