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Key Documents

L5024

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O127:B8

purified by ion-exchange chromatography, TLR ligand tested

Synonyme(s) :

LPS

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About This Item

Numéro CE :
297-473-0
Numéro MDL:
MFCD00212998
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25

Source biologique

Escherichia coli (O127:B8)

Niveau de qualité

200

Forme

lyophilized powder

Produit purifié par

ion-exchange chromatography

Impuretés

<1% Protein
<1% RNA

Couleur

white to yellow cast

Solubilité

water: soluble

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Catégories apparentées

Description générale

This product is extracted from E. coli serotype O127:B8 and purified by ion exchange. The source strain is ATCC 12740. This LPS serotype has been used in the study of septic shock and to induce NOS in murine macrophages and PAF synthesis in rat glomerular masangial cells.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Actions biochimiques/physiologiques

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Notes préparatoires

The product is soluble in water (5 mg/mL) or cell culture medium (1 mg/mL) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/mL) has been achieved in aqueous saline after vortexing and warming to 70-80 °C. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Skull and crossbones
GHS06

Mention d'avertissement

Danger

Mentions de danger

H300

Classification des risques

Acute Tox. 2 Oral

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Mingju Cao et al.
Frontiers in cellular neuroscience, 9, 294-294 (2015-08-25)
Neuroinflammation in utero may result in life-long neurological disabilities. The molecular mechanisms whereby microglia contribute to this response remain incompletely understood. Lipopolysaccharide (LPS) or saline were administered intravenously to non-anesthetized chronically instrumented near-term fetal sheep to model fetal inflammation in
Kristen R Hollinger et al.
Journal of Alzheimer's disease : JAD, 77(1), 437-447 (2020-07-18)
Given the emergent aging population, the identification of effective treatments for Alzheimer's disease (AD) is critical. We investigated the therapeutic efficacy of JHU-083, a brain-penetrable glutamine antagonist, in treating AD using the humanized APOE4 knock-in mouse model. Cell culture studies
M Cortes et al.
Scientific reports, 7(1), 10645-10645 (2017-09-08)
Neuroinflammation in utero may result in life-long neurological disabilities. Microglia play a pivotal role, but the mechanisms are poorly understood. No early postnatal treatment strategies exist to enhance neuroprotective potential of microglia. We hypothesized that agonism on α7 nicotinic acetylcholine
Mingju Cao et al.
Frontiers in immunology, 10, 1063-1063 (2019-05-31)
Neuroinflammation in utero may result in lifelong neurological disabilities. Astrocytes play a pivotal role in this process, but the mechanisms are poorly understood. No early postnatal treatment strategies exist to enhance neuroprotective potential of astrocytes. We hypothesized that agonism on
Manman Geng et al.
Clinical immunology (Orlando, Fla.), 220, 108579-108579 (2020-09-01)
Endoplasmic reticulum (ER) stress associated proteins contribute to the pathogenesis of rheumatoid arthritis (RA) through affecting synoviocyte proliferation and proinflammatory cytokine production. The role of DERL3, an ER-associated degradation component, in joint inflammation of RA was explored. Synovial tissues from
Afficher tous les articles scientifiques apparentés

Articles

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Contenu apparenté

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

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