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L3892

Sigma-Aldrich

Lectine de Triticum vulgaris (blé)

peroxidase conjugate, lyophilized powder

Synonyme(s) :

Agglutinine de germe de blé, WGA

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

Triticum vulgaris

Niveau de qualité

200

Conjugué

peroxidase conjugate

Pureté

≥85% protein basis (Warburg-Christian)

Forme

lyophilized powder

Puissance

<40 μg/mL agglutination activity

peroxidase activity

50-200 units/mg protein

Composition

Protein, ~90% modified Warburg-Christian

Température de stockage

−20°C

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Application

Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Actions biochimiques/physiologiques

La WGA n′est pas spécifique à un groupe sanguin, mais elle présente une affinité pour les résidus N-acétyl-β-D-glucosaminyl et les oligomères du type N-acétyl-β-D-glucosamine. La WGA ne contient aucun glucide lié à une protéine.

Conditionnement

Package size based on protein content.

Définition de l'unité

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Forme physique

Contains citrate buffer

Notes préparatoires

Les conjugués sont préparés à partir d′une lectine purifiée par affinité.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Hugo Aguilar-Díaz et al.
PLoS neglected tropical diseases, 4(2), e607-e607 (2010-02-20)
Inhibition of encystment can be conceived as a potentially useful mechanism to block the transmission of Entamoeba histolytica under natural conditions. Unfortunately, amoeba encystment has not been achieved in vitro and drugs inhibiting the formation of cysts are not available.
Xinyu Qi et al.
Journal of bone and joint infection, 6(7), 241-253 (2021-07-16)
The high antibiotic tolerance of Staphylococcus aureus biofilms is associated with challenges for treating periprosthetic joint infection. The toxin-antitoxin system, YefM-YoeB, is thought to be a regulator for antibiotic tolerance, but its physiological role is unknown. The objective of this
Qian Guo et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 36(10), 2065-2080 (2021-06-23)
ATP-citrate lyase (ACLY), generating most of the nucleocytosolic acetyl coenzyme A (acetyl-CoA) for histone acetylation, links cell metabolism to epigenetic regulation. Recent investigations demonstrated that ACLY activated by metabolic reprogramming played an essential role in both M1 and M2 macrophage
Qian Guo et al.
The Journal of biological chemistry, 101775-101775 (2022-03-09)
It's widely accepted that increasing mitochondrial respiration plays a pivotal role during osteoclastogenesis. Mitochondrial pyruvate carrier (MPC) is the key transporter that links glycolysis to mitochondrial respiration but little is known about its role during osteoclastogenesis. Our goal was to
Xiao Lin et al.
Frontiers in cell and developmental biology, 10, 796227-796227 (2022-05-07)
Osteoporosis, characterized by the destruction of bone resorption and bone formation, is a serious disease that endangers human health. Osteoporosis prevention and treatment has become one of the important research contents in the field of medicine. Acacetin, a natural flavonoid
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