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L1394

Sigma-Aldrich

Lectine de Triticum vulgaris (blé)

agarose conjugate, saline suspension

Synonyme(s) :

Agglutinine de germe de blé, WGA

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Conjugué

agarose conjugate

Niveau de qualité

200

Forme

saline suspension

Ampleur du marquage

~6 mg per mL

Matrice

4% agarose

Activation de la matrice

cyanogen bromide

Espaceur de matrice

1 atom

Taille des particules

45-165 μm

Capacité

≥0.5 mg/mL binding capacity (ovomucoid)

Température de stockage

2-8°C

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Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.

Actions biochimiques/physiologiques

La WGA n′est pas spécifique à un groupe sanguin, mais elle présente une affinité pour les résidus N-acétyl-β-D-glucosaminyl et les oligomères du type N-acétyl-β-D-glucosamine. La WGA ne contient aucun glucide lié à une protéine.

Forme physique

Suspension in 0.9% NaCl containing 15 mM Sodium azide.

Notes préparatoires

Les conjugués sont préparés à partir d′une lectine purifiée par affinité.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Bo F Hansen et al.
PloS one, 7(5), e34274-e34274 (2012-05-17)
There is controversy with respect to molecular characteristics of insulin analogues. We report a series of experiments forming a comprehensive characterisation of the long acting insulin analogues, glargine and detemir, in comparison with human insulin, IGF-1, and the super-mitogenic insulin
Liheng Yang et al.
The Journal of biological chemistry, 291(8), 3905-3917 (2015-12-20)
The normal cellular prion protein (PrP) is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein. However, in pancreatic ductal adenocarcinoma cell lines, such as BxPC-3, PrP exists as a pro-PrP retaining its glycosylphosphatidylinositol (GPI) peptide signaling sequence. Here, we report the identification
María-Ángeles Cortés-Gómez et al.
International journal of molecular sciences, 24(2) (2023-01-22)
In Alzheimer's disease (AD), the reduction in acetylcholinesterase (AChE) enzymatic activity is not paralleled with changes in its protein levels, suggesting the presence of a considerable enzymatically inactive pool in the brain. In the present study, we validated previous findings
D L Christie et al.
The Biochemical journal, 270(1), 57-61 (1990-08-15)
Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The
Cynthia Tang et al.
Communications biology, 4(1), 161-161 (2021-02-07)
Mutations in CLN3 lead to photoreceptor cell loss in CLN3 disease, a lysosomal storage disorder characterized by childhood-onset vision loss, neurological impairment, and premature death. However, how CLN3 mutations cause photoreceptor cell death is not known. Here, we show that

Articles

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Protocoles

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

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