K4002

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

• Synonyme(s) : Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
• Code UNSPSC : 12161702
• Nomenclature NACRES : NA.75

Propriétés

• Niveau de qualité: 500
• Forme: powder
• Technique(s): cell culture | mammalian: suitable
• pH: 6.1-6.7
• Solubilité: water: 9.5 g/L, clear
• Température de stockage: 2-8°C

Description

Description générale

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Application

Krebs-Ringer Bicarbonate Buffer has been used:

• as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
• in the pre-perifusion for islet viability and functionality studies
• in pituitary bioassay

Quantité

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 1.26 g/L sodium bicarbonate.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable