H5912

Anti-phospho-Histone H2AX (pSer¹³⁹) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : H2Ax Antibody, H2Ax Antibody - Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit, Anti-H2AXS139p
• Numéro MDL: MFCD04974783
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 15 kDa
• Espèces réactives: mouse, human
• Technique(s): microarray: suitable; western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine
• Numéro d'accès UniProt: P16104
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: phosphorylation (pSer139)
• Informations sur le gène: human ... H2AFX(3014) ; mouse ... H2afx(15270)

Description

Description générale

H2AX comprises 2-25% of the total H2A complement in human cells, whereas in the budding yeast it constitutes virtually all of the H2A molecules. It has N-terminal and C-terminal region with a central globular domain. The C-terminal region has sites for modification including acetylation, biotinylation and phosphorylation.

Immunogène

The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.

synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer¹³⁹).

Application

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting.

Actions biochimiques/physiologiques

H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA. Ataxia-telangiectasia-mutated (ATM) is the major kinase involved in the phosphorylation of H2AX in response to DNA double-strand breaks. H2AX knockout mice are radiation sensitive and show retarted growth. They also are immune deficient with mutant males are infertile..

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable