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H2519

Sigma-Aldrich

Heparinase I from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

Synonyme(s) :

Heparin lyase I, Heparinase

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About This Item

Numéro CAS:
9025-39-2
Numéro de classification (Commission des enzymes):
4.2.2.7 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131317
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Conjugué

conjugate (Glucosaminoglycan)

Niveau de qualité

200

Forme

lyophilized solid

Activité spécifique

≥200 units/mg protein (enzyme + BSA)

Poids mol.

42.8 kDa

Application(s)

diagnostic assay manufacturing

Température de stockage

−20°C

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Application

Heparinase I from Flavobacterium heparinum has been used:
  • in the digestion of plasma samples
  • to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia
  • in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells

Actions biochimiques/physiologiques

Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.

Définition de l'unité

One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro
Yan Y, et al.
Journal of Microbiological Methods, 61(1), 127-130 (2005)
HeparinHeparin - A Century of Progress, 273-273 (2012)
Wataru Hashimoto et al.
Biochemistry, 53(4), 777-786 (2014-01-21)
Pedobacter heparinus (formerly known as Flavobacterium heparinum) is a typical glycosaminoglycan-degrading bacterium that produces three heparin lyases, Hep I, Hep II, and Hep III, which act on heparins with 1,4-glycoside bonds between uronate and amino sugar residues. Being different from
Cardiopulmonary Bypass: Principles and Practice, 509-509 (2008)
M E Casey et al.
Anaesthesia, 68(1), 91-96 (2012-10-16)
Paediatric patients who require anticoagulation with therapeutic doses of low-molecular weight heparin are at risk of having a residual anticoagulant effect at the time of surgery, even if managed according to current peri-operative guidelines. Testing for residual effect is not
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