GUSS
β-Glucuronidase Reporter Gene Staining Kit
Synonyme(s) :
β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.32
Produits recommandés
Utilisation
kit sufficient for 100 histochemical assays
Niveau de qualité
Technique(s)
microbe id | staining: suitable
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.
Application
The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Caractéristiques et avantages
- Histochemical staining of plant tissues expressing the E. coli GUS enzyme
- Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
- GUS does not interfere with plant cell function or viability
Autres remarques
The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.
Composants de kit seuls
Réf. du produit
Description
- 2× Fixation Buffer 25 mL
- 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
- Reagent A for gus staining kit 50 mL
- Reagent B for gus staining kit 200 μL
- Reagent C for gus staining kit 200 μL
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
11 - Combustible Solids
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We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We
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