GE28-9782-45

HiScreen^™, DEAE Fast Flow

Cytiva 28-9782-45

• Code UNSPSC : 47101511
• Nomenclature NACRES : NA.56

Propriétés

• ligand: diethylaminoethyl
• Description: Ion Exchanger Type (value)
• Forme: prepacked column
• Réticulation: cross-linked
• Conditionnement: pkg of 1 ea
• Fabricant/nom de marque: Cytiva 28-9782-45
• Conditions de stockage: (20% Ethanol)
• Paramètres: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• Technique(s): liquid chromatography (LC): suitable
• Taille du lit: 7.7 mm × 100 mm
• Volume du lit: 4.7 mL
• D.I. de la colonne: 7.7 mm
• Matrice: 6% cross-linked agarose
• Groupe de la matrice active: N⁺(C₂H₅)₂H functional group
• Taille des particules: 45-165 μm
• Diamètre moyen: 90 μm
• cleaning: 2-14
• working range: 2-12
• Adéquation: suitable for bioprocess medium
• Technique de séparation: anion exchange

Description

Description générale

HiScreen^™ DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Application

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen^™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen^™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Caractéristiques et avantages

• DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose^™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
• Prepacked, ready-to-use columns for convenient process development optimization.
• 10 cm bed height of HiScreen^™ columns is designed to allow method optimization and parameter screening.
• Easily connected in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption.
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiScreen is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids