GE28-9537-67
HiTrap® TALON® Crude
Cytiva 28-9537-67, pack of 5 × 5 mL
Synonyme(s) :
Crude TALON Column, HiTrap TALON, TALON Crude Column
About This Item
Produits recommandés
ligand
TALON® Superflow Cobalt-IMAC
Conditionnement
pack of 5 × 5 mL
Fabricant/nom de marque
Cytiva 28-9537-67
Paramètres
1 ml/min - 4 ml/min flow rate
3 bar
D.I. de la colonne
7 mm
Matrice
6% cross-linked agarose
Taille des particules
60-160 μm
cleaning
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
Capacité
≥20 mg binding capacity (histidine-tagged protein)
Température de stockage
2-8°C
Description générale
Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Talon® Superflow and prepacked HiTrap® Talon® crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.
Caractéristiques et avantages
- Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
- Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
- HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.
Stockage et stabilité
Remarque sur l'analyse
Autres remarques
Informations légales
Produit(s) apparenté(s)
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
Protocoles
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
How to isolate proteins from inclusion bodies using Cytiva products.
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