GE28-9365-51
Hisprep™ Fast Flow 16/10
Cytiva 28-9365-51
Synonyme(s) :
16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column
About This Item
Produits recommandés
Conditionnement
pkg of 1 ea
Fabricant/nom de marque
Cytiva 28-9365-51
Paramètres
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
Taille du lit
16 mm × 100 mm
Volume du lit
20 mL
D.I. de la colonne
16 mm
Matrice
6% cross-linked agarose
Taille des particules
45-165 μm
Diamètre moyen
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
Capacité
~40 mg binding capacity(histidine-tagged protein)
Adéquation
suitable for bioprocess medium
Catégories apparentées
Description générale
HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
Caractéristiques et avantages
- Optimized for convenient scale-up purification of histidine-tagged proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible leakage of Ni2+.
- High binding capacity, approx. 40 mg/mL medium.
- Convenient and time-saving 20 mL prepacked HiPrep™ format.
Stockage et stabilité
Remarque sur l'analyse
Autres remarques
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
Protocoles
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
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