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GE17-5279-01

Glutathione Sepharose™ High Performance

Cytiva 17-5279-01, pack of 25 mL

Synonyme(s) :

Glutathione Sepharose Column

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

glutathione

Conditionnement

pack of 25 mL

Fabricant/nom de marque

Cytiva 17-5279-01

Matrice

highly cross-linked 6% agarose

Taille moy. des particules

34 μm

cleaning

3-12

working range

3-12

Capacité

>7 mg/mL binding capacity(medium)

Description générale

Glutathione Sepharose™ High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.

Glutathione Sepharose™ High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function.

Caractéristiques et avantages

  • 34 μm bead size for improved efficiency and resolution
  • Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins
  • Mild conditions during the purification process preserves protein antigenicity and function

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

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Protocoles

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

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