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GE17-1287-10

Anx Sepharose™ Fast Flow High Sub

Cytiva 17-1287-10, pack of 25 mL

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About This Item

Code UNSPSC :
47101511
Nomenclature NACRES :
NA.56

ligand

diethylaminopropyl

Description

Ion Exchanger Type (value)

Conditionnement

pack of 25 mL

Fabricant/nom de marque

Cytiva 17-1287-10

Matrice

4% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

2-14

working range

3-13

Adéquation

suitable for bioprocess medium

Description générale

ANX Sepharose™ 4 Fast FLow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins.

Application

ANX Sepharose™ Fast FLow (high sub) is part of the Sepharose™ Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. ANX Sepharose™ 4 Fast FLow (high sub) is a weak anion exchanger medium with tertiary amine groups attached to the base matrix Sepharose™ 4 Fast FLow via ether linkage and a hydrophilic spacer arm. The diethylaminopropylgroup is coupled in a mode that precludes formation of quaternary groups, which are typically seen on traditional DEAE media, resulting in a truly weak anion exchanger. The base matrix, Sepharose™ 4 Fast Flow, is a highly cross-linked, 4% Agarose derivative with high chemical and physical stability and broad separation range. ANX Sepharose™ 4 Fast FLow (high sub) has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.

As member of the BioProcess media range, ANX Sepharose™ 4 Fast FLow (high sub) meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caractéristiques et avantages

  • Weak anion exchanger with different selectivity compared with traditional ion exchangers.
  • Applicable for separation of high molecular weight Proteins
  • Developed in co-operation with leading large-scale pharmaceutical manufacturers
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocoles

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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