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Key Documents

GE17-0444-01

Lentil Lectin-Sepharose™ 4B 25 mL

Cytiva 17-0444-01

Synonyme(s) :

Sepharose 4B

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

lentil lectin

Conditionnement

pkg of 25 mL

Fabricant/nom de marque

Cytiva 17-0444-01

Conditions de stockage

(20% Ehtanol, PBS pH 7)

Matrice

4% agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

3-12

working range

3-10

Température de stockage

2-8°C

Catégories apparentées

Description générale

Lentil Lectin Sepharose™ media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A.

Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose™ 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.

Caractéristiques et avantages

  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
  • Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
  • Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
  • Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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Articles

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

Purification or Removal of Glycoproteins and Polysaccharides

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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