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Key Documents

GE11-0034-95

HiTrap® MabSelect SuRe™

Cytiva 11-0034-95, pack of 5 × 5 mL

Synonyme(s) :

prepacked columns, MabSelecT columns

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

alkali-stabilized protein A-derived (E. coli)

Conditionnement

pack of 5 × 5 mL

Fabricant/nom de marque

Cytiva 11-0034-95

Conditions de stockage

(20% Ehtanol)
 (20% Ethanol)

Paramètres

flow rate
42 psi

Taille du lit

16 mm × 25 mm

Volume du lit

5 mL

D.I. de la colonne

16 mm

Matrice

highly cross-linked agarose

Taille moy. des particules

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-13.7

working range

3-12

Capacité

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

Adéquation

suitable for bioprocess medium

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ SuRe
HiTrap® MabSelect™ SuRe™ columns are prepacked with MabSelect™ SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).

HiTrap® MabSelect™ columns are prepacked with MabSelect™ a bioprocess medium for capuring of Mabs from large sample volumes.

Caractéristiques et avantages

  • Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
  • Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva
SuRe is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocoles

Performing a Purification of IgG Antibodies with MabSelect Sure

This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique