G5507

Galactosyltransferase from bovine milk

lyophilized powder

• Synonyme(s) : Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase
• Numéro CAS: 9031-68-9
• Numéro de classification (Commission des enzymes): 2.4.1.22 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131122
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: 3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)
• Composition: Protein, 35-65% modified Warburg-Christian
• Impuretés: ≤10 % protein α-lactalbumin
• Température de stockage: −20°C

Description

Description générale

Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.

Application

Galactosyltransferase from bovine milk has been used:

• in the generation of hyper-galactosylated variant of IgG1
• in β -1,4-galactosyltransferase assay rice root cell extracts
• in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Actions biochimiques/physiologiques

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.

Galactosyltransferase from bovine milk requires Mn²⁺ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various disease states.Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.

Liaison

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Définition de l'unité

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Forme physique

Lyophilized powder containing Tris, EDTA, and (NH₄)₂SO₄

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)