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Principaux documents

G3293

Supelco

Réactif de dosage du glucose

for use with enzymatic assay kits GAHK20, SCA20, FA20, SA20

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About This Item

Code UNSPSC :
12164500
Nomenclature NACRES :
NA.84
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

Classe(s) chimique(s) de l'analyte

sugars (glucose)

Technique(s)

photometry: suitable

Impuretés

≤0.005% Creatine phosphokinase, glutathione reductase, myokinase, NADH-oxidase,NADPH oxidase, phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase
<0.01% ATP-ase, myokinase

Application(s)

food and beverages
general analytical

Température de stockage

2-8°C

Spécificité

< Activité < 0,05 % résultant de réactions croisées avec les substrats suivants : fructose, dextran, sédoheptulose, arabinose, galactose, xylose, tréhalose, mannose, sorbitol, +/- fucose ou ribose.

Application

Pour la détermination quantitative du glucose dans les aliments et autres produits avec le test enzymatique à l′hexokinase-glucose 6-phosphate déshydrogénase.
Reconstituer dans de l′eau déionisée selon le volume indiqué sur le conditionnement (20 ou 50 ml).

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Protocoles

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

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Questions

1–6 of 6 Questions  
  1. For how many tests this reagent will be sufficient?

    1 answer
    1. This product is available in a 20 mL or 50 mL package size. The entire vial should be reconstituted in DI water as indicated by the package size. Each test requires 1 mL of this reagent. The 20 mL package size is sufficient to perform 20 test and the 50 mL pack size is sufficient to perform 50 tests.

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  2. How much of the initial must be reconstituted in 20 ml of DI water?

    1 answer
    1. The entire vial should be reconstituted to the volume of the package size. The G3293-20 mL with 20 mL of DI water and the G3293-50 mL with 50 mL of DI water.

      Helpful?

  3. Is magnesium required for Product G3293, Glucose Assay Reagent?

    1 answer
    1. Magnesium, an activator of hexokinase, is included in the assay reagent. There is no need to add a magnesium salt to the reagent before use.

      Helpful?

  4. Is it possible to adapt Product G3293, Glucose Assay Reagent, for use with a 96 well plate reader?

    1 answer
    1. In principle, it should be possible to adapt assay procedures using this reagent to 96-well plate readers. Volumes of reagent, water, standard, and unknown should be reduced to fit the capacity of the test wells, and kept in the same proportions as those used with a spectrophotometer. The volume reduction will likely be 1:4 or 1:5. With the shorter "pathlength" of a multiwell plate, the sensitivity of the assay would likely decrease.

      Helpful?

  5. What is the detection limit of Product G3293, Glucose Assay Reagent?

    1 answer
    1. This reagent should be able to detect glucose down to approximately 0.01 mg/mL using a spectrophotometer with a 1 cm path length.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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