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G2501

Sigma-Aldrich

L-Glutamic Dehydrogenase from bovine liver

Type I, ammonium sulfate suspension, ≥40 units/mg protein

Synonyme(s) :

L-GLDH, L-Glutamate:NAD[P]+ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver

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About This Item

Numéro CAS:
9029-12-3
Numéro de classification (Commission des enzymes):
1.4.1.3 (BRENDA, IUBMB)
Numéro CE :
232-848-4
Numéro MDL:
MFCD00131461
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Type

Type I

Niveau de qualité

200

Forme

ammonium sulfate suspension

Activité spécifique

≥40 units/mg protein

Poids mol.

310-350 kDa

Numéro d'accès UniProt

P00366

Température de stockage

2-8°C

Informations sur le gène

cow ... GLUD1(281785)

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Actions biochimiques/physiologiques

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.
Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:
  • It has a reversible concentration-dependent association, producing higher molecular weight forms.
  • Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
  • Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Définition de l'unité

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

Forme physique

Suspension in 2.0 M (NH4)2SO4 solution

Remarque sur l'analyse

Protein determined by biuret

Substrat

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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